Amersham™ Typhoon™ Laser Scanner from Cytiva

Product Specs
ItemAmersham™ Typhoon™ Laser Scanner
CompanyCytiva Life Sciences
Price Get Pricing Get Quote for this Product
Catalog Number29187191 / 29187193 / 29187194 / 29238583 / 29264463
Dimensions(WxDxH) 900 x 800 x 400 mm
QuantityEA
ApplicationsIdeal for versatile imaging and precise quantitation of fluorescent, colorimetric, and radiolabeled nucleic acids and proteins, phosphor imaging, fluorescence red, green, blue, and NIR, OD, and sample types (imaging plates, gels, plates, tissue slides, membranes) — from narrow to very large samples.
TypeLaser-Scanner
VoltageAC100—240V~, Voltage variation±10%
LasersLD488, SHG532, LD635, LD685, LD785
Scan Area40 x 46 cm
Pixel Sizes10, 25, 50, 100 or 200 µm and a 1000 µm pre-scan
FilterIP Fltr 390BP, Cy2 Fltr 525BP20, Cy3 Fltr 570BP20, Cy5 Fltr 670BP30, IRshort Fltr 720BP20, IRlong Filtr 825BP30

Cytiva Life Sciences

(1) Human nucleolar protein 7 (NOL7) is required for early pre-rRNA accumulation and pre-18S rRNA processingRNA BiologyDecember 31, 2023Mason A. McCool, Carson J. Bryant, Hannah Huang, Lisa M. Ogawa, Katherine I. Farley-Barnes, Samuel B. Sondalle, Laura Abriola, Yulia V. Surovtseva, Susan J. Baserga5’-AAGGGGTCTTTAAACCTCCGCGCCGGAACGCGCTAGGTAC-3’) and detected by phosphorimager (Amersham™ Typhoon™, 29187194). Images were analysed using ImageJ, ratio-analysis of multiple precursors (RAMP) was performed [16]. BioAnalyzer For

(2) Hydrolysis of Oligodeoxyribonucleotides on the Microarray Surface and in Solution by Catalytic Anti-DNA Antibodies in Systemic Lupus ErythematosusCurr Issues Mol BiolDecember 8, 2023Tatiana S. Novikova, Evgeny A. Ermakov, Elena V. Kostina, Alexander N. Sinyakov, Alexey E. Sizikov, Georgy A. Nevinsky, Valentina N. BunevaELF-8 (DNA-Technology LLC, Moscow, Russia). The results of gel electrophoresis were recorded using an Amersham Typhoon laser scanner (Cytiva, Uppsala, Sweden). To determine the relative level of hydrolysis (%), the intensities of the

(3) Bacillus subtilis NrnB is expressed during sporulation and acts as a unique 3′-5′ exonucleaseNucleic Acids ResearchOctober 13, 2023Tanner M Myers, Shakti Ingle, Cordelia A Weiss, Holger Sondermann, Vincent T Lee, David H Bechhofer, Wade C Winkleryield was determined by running an aliquot of the reaction on denaturing 20% PAGE and using a Cytiva Amersham Typhoon™ laser scanner platform. The intensity of radiolabeled c-di-AMP relative to the remaining ATP in the reaction was quantified

(4) GFP fusions of Sec-routed extracellular proteins in Staphylococcus aureus reveal surface-associated coagulase in biofilmsMicrobial CellJuly 3, 2023Dominique C. S. Evans, Amanda B. Khamas, Lisbeth Marcussen, Kristian S. Rasmussen, Janne K. Klitgaard, Birgitte H. Kallipolitis, Janni Nielsen, Daniel E. Otzen, Mark C. Leake, Rikke L. Meyergel (Mini-PROTEAN TGX, 4561086, Bio-Rad). Fluorescence was detected in the Amersham Typhoon Scanner (29187191, Cytiva) with a 488 nm excitation and 510 nm emission. A His-tagged GFP (14-392, Sigma-Aldrich) was

(5) Protocol for monitoring and analyzing single nucleotide incorporation by S. cerevisiae RNA polymerasesSTAR ProtocolsJune 1, 2023Ruth Q. Jacobs, Nathan F. Bellis, Aaron L. Lucius, David A. SchneiderScientific,29175523 GE Healthcare image eraser,GE,Model#810-UNV GE Amersham Typhoon 5,Cytiva,Part#29187191 "Laboratory Dual Input, Multi-Parameter Meter - pH, Conductivity, Optical Dissolved Oxygen, ORP, and

(6) Noncatalytic regulation of 18S rRNA methyltransferase DIMT1 in acute myeloid leukemiaGenes & DevelopmentApril 1, 2023Yulia Gonskikh, Julian Stoute, Hui Shen, Krista Budinich, Bianca Pingul, Kollin Schultz, Heidi Elashal, Ronen Marmorstein, Junwei Shi, Kathy Fange Liuglycerol) and run at 120 V for 35 min at 4°C. The gel was imaged using an Amersham Typhoon 5 (Cytiva 29187191) fluorescent scan with a 473-nm laser. Quantification was carried out using Fiji ImageJ to quantify

(7) CRISPR/Cas9-based Engineering of Immunoglobulin Loci in Hybridoma CellsBIO-PROTOCOLFebruary 20, 2023Camille Le M. Le Gall, Felix L. Fennemann, Johan M. S. van der Schoot, Ferenc A. Scheeren, Martijn VerdoesBiosciences, catalog number: 663518)Fluorescent scanner, e.g., Amersham TyphoonTM 5 (Cytiva, catalog number: 29187191)Sanger Sequencing Service Software Snapgene (from Insightful Science; available at snapgene.com)FlowJoTM

(8) NrnA is a 5′-3′ exonuclease that processes short RNA substrates in vivo and in vitroNucleic Acids ResearchDecember 7, 2022Cordelia A Weiss, Tanner M Myers, Chih Hao Wu, Conor Jenkins, Holger Sondermann, Vincent T Lee, Wade C Winkleryield was determined by running an aliquot of the reaction on denaturing 20% PAGE and using a Cytiva Amersham Typhoon™ laser scanner platform. The intensity of radiolabeled c-di-AMP relative to the remaining ATP in the reaction was quantified

(9) Zhilong Huoxue Tongyu Capsules Ameliorate Early Brain Inflammatory Injury Induced by Intracerebral Hemorrhage via Inhibition of Canonical NFкβ Signalling PathwayFrontiers in PharmacologyMarch 31, 2022Maryam Mazhar, Guoqiang Yang, Linshen Mao, Pan Liang, Ruizhi Tan, Li Wang, Houping Xu, Luyin Yang, Wei Ren, Sijin Yang1 h. Then, membranes were washed with TBST 5 min x3. Fluorescent detection was performed using the Amersham Typhoon™ laser scanner (Cytiva, USA). Protein levels were normalized with respect to GAPDH and quantified using the ImageJ

(10) How to build a ribosome from RNA fragments in Chlamydomonas mitochondriaNature CommunicationsDecember 9, 2021Florent Waltz et al.0.1% SDS at the hybridization temperature. For each specific probe, the signal was detected with the Amersham Typhoon laser scanner (Amersham). For the quantitative real-time RT-PCR analyses, RNAs were treated with RQ1 RNase-Free DNase

(11) Designed architectural proteins that tune DNA looping in bacteriaNucleic Acids ResearchOctober 11, 2021David H Tse, Nicole A Becker, Robert T Young, Wilma K Olson, Justin P Peters, Tanya L Schwab, Karl J Clark, L James Maherin blocking buffer for 1 h at room temperature with gentle rocking. Membranes were analyzed with an Amersham Typhoon laser-scanner platform (Cytiva) using IRshort and IRlong methods. Uncropped images are shown in Supplementary Figure

(12) Development of Keratinocyte Cell Lines Containing Extrachromosomal Human Papillomavirus GenomesCurrent ProtocolsSeptember 8, 2021Tami L. Coursey, Alison A. McBrideBAS Storage Phosphor Screens 28956480) Phosphorimager (e.g. Cytiva Amersham Typhoon phosphorimager 29238583) Shaker or rocker Tabletop centrifuge Spectrophotometer, such as Nanodrop DNA sample digestion NEB

(13) Single-molecule studies reveal branched pathways for activator-dependent assembly of RNA polymerase II pre-initiation complexesMolecular CellSeptember 1, 2021Inwha Baek, Larry J. Friedman, Jeff Gelles, Stephen Buratowski2015)",https://github.com/gelles-brandeis/CoSMoS_Analysis Other,Other,Other Phosphoscanner/fluorescence imager Amersham Typhoon 5,GE healthcare,Model: 29187191 Homogenizer,Wheaton,Cat#62400–802 ,, ,, ,,

(14) Neurodegenerative VPS41 variants inhibit HOPS function and mTORC1‐dependent TFEB/TFE3 regulationEMBO Molecular MedicineMay 7, 2021Reini E N van der Welle et al.followed by 2 washing steps with PBS and 1 washing step with MiliQ. Membranes were scanned using the Amersham™ Typhoon™ Laser Scanner (GE Healthcare Life Sciences). Plasmids The GFP‐VPS41 constructs were cloned from hVPS41 cDNA (Origene)

(15) Nonenzymatic RNA-templated Synthesis of N3′→P5′ Phosphoramidate DNABIO-PROTOCOLSeptember 5, 2020Derek K O'Flaherty, Lijun Zhou, Jack W SzostakElectrophoresis) 27. Amersham Typhoon RGB Biomolecular Imager (GE Healthcare Life Sciences, catalog number: 29187193) 28. Nuclear Magnetic Resonance (NMR, 400MHz Variant) Software 1. ChemDraw (PerkinElmer) 2. ImageQuantTM

(16) Identification of Footprints of RNA:Protein Complexes via RNA Immunoprecipitation in Tandem Followed by Sequencing (RIPiT-Seq)Journal of Visualized ExperimentsJuly 30, 2019Lauren Woodward, Pooja Gangras, Guramrit Singhcompatible with the Illumina sequencing platform Typhoon 5 Bimolecular Imager,GE Healthcare Life Science,29187191,

(17) The Dolphin Proline-Rich Antimicrobial Peptide Tur1A Inhibits Protein Synthesis by Targeting the Bacterial RibosomeCell Chemical BiologyMay 1, 2018Mario Mardirossian et al.M1000,Tecan,N/A M-110L Microfluidizer Processor,Microfluidics,N/A Typhoon FLA9500,GE Healthcare,Catalog # 29187191 Protino Ni-NTA agarose beads,Macherey-Nagel,Catalog # 745400 Superdex HiLoad S75 16/600,GE Healthcare,Catalog

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